Increase of ruminal fiber digestion by cellobiose and a twin strain of Saccharomyces cerevisiae live cells in vitro
2006
Lila, Z.A.(Tokyo Univ. of Agriculture and Technology, Fuchu (Japan). Faculty of Agriculture) | Mohammed, N. | Takahashi, T. | Tabata, M. | Yasui, T. | Kurihara, M. | Kanda, S. | Itabashi, H.
This experiment was designed to investigate the effects of different concentrations of cellobiose (CB) or a twin strain of Saccharomyces cerevisiae live cells (YST) (20, 40 and 60 mg/60 mL), and CB + YST (60 + 20, 60 + 40, 60 + 60 mg/60 mL) on mixed ruminal microorganism fermentation in vitro. Ruminal fluid was collected from a cow, mixed with phosphate buffer (1:2) and incubated (60 mL) anaerobically at 38 deg C for 24 h with or without supplement plus 400 mg (dry matter [DM] basis) substrate (hay plus concentrate, 1.5:1). The medium pH numerically decreased with CB and CB + YST, but was unchanged with YST. The total volatile fatty acid and proportion of propionate increased (P0.05) in all cases. The proportion of acetate decreased (P0.05) with CB, and CB + YST, but increased (P0.05) with YST and that of butyrate increased (P0.05) with CB and CB + YST, but decreased (P0.05) with YST. Ammonia-N decreased (P0.05) with CB and CB + YST, but was unchanged with YST. The number of protozoa was unchanged, and that of cellulolytic bacteria increased (P0.05) in all cases. Total gas production increased (P0.05) in all cases. Methane decreased, hydrogen was unchanged by YST and both gases were unchanged by CB and CB + YST. The in vitro disappearance of DM and neutral detergent fiber increased (P0.05) by 11.2% and 8.9%, 9% and 8.5%, and 12.1% and 10.2% in the case of CB, YST and CB + YST, respectively. Therefore, the dietary supplementation of CB and/or YST may improve ruminal fermentation and digestibility.
Показать больше [+] Меньше [-]