Allele frequencies of TYR and MC1R in Chinese native cattle
Gan, H.Y.(Shandong Academy of Agricultural Science, Jinan (China). Dairy Cattle Research Center) | Li, J.B. | Wang, H.M. | Gao, Y.D. | Liu, W.H. | Li, J.P. | Zhong, J.F.
In order to estimate the influence of TYR and MC1R on the color of the cattle hide, the MC1R and TYR in Luxi Yellow, Bohai Black, China Holstein black-white and China Holstein red-white cattle(20 animals of each of the four breeds) were sequenced. The comparison of TYR among the four hide color phenotypes revealed no sequence difference. The sequences of the MC1R coding region revealed three alleles (Esup(D), Esup(+) and e), which were previously reported. Furthermore, we found an important single nucleotide polymorphism at 725 position of the MC1R coding region, which may help in cattle breed identification. A polymerase chain reaction-restriction fragment length polymorphism was performed to investigate the gene frequencies of the four breeds. Most China Holstein black-white cattle had Esup(D) and Esup(+) alleles (Esup(D)=0.12, Esup(+)=0.80) and no homozygous e/e and most Bohai Black cattle had Esup(D) and Esup(+) alleles(Esup(D)=0.52, Esup(+)=0.47). Therefore it is consistent with the hypothesis that Esup(D) and Esup(+) induce black pigment synthesis. On the other hand, most of the China Holstein red-white cattle and Luxi Yellow cattle had the e allele (e/e=0.95). Unexpectedly, the Esup(+)/e genotype was present in China Holstein red-white cattle and Luxi Yellow cattle.
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