Induction of a large deletion including the waxy locus by thermal nuetron irradiation in rice [Oryza sativa]
2007
Kawakami, S.(National Inst. of Agrobiological Sciences, Tsukuba, Ibaraki (Japan)) | Kadowaki, K. | Morita, R. | Nishimura, M.
Sequence rearrangement of rice waxy (wx) mutant N26 induced by thermal neutron irradiation was analyzed by PCR amplification and subsequent DNA sequencing. We found that the genome region of Norin 8, including the Wx locus, had been deleted, corresponding to 39 867 nucleotides for the japonica cultivar Nipponbare. Sequence analysis suggested that a short homologous sequence was used for rejoining without any addition of nucleotides. This is the first mutant in which a complete loss of the Wx locus was induced by thermal neutron irradiation in rice.
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