Toxicity Evaluation of Organochloride Pesticide, Endosulfan and its Metabolites Using Microalgae
2006
Sohn, H.Y. (Andong National University, Andong, Republic of Korea), E-mail: [email protected] | Kum, E.J. (Andong National University, Andong, Republic of Korea) | Kim, J.S. (Andong National University, Andong, Republic of Korea) | Lee, J.B. (Andong National University, Andong, Republic of Korea) | Kwon, G.S. (Andong National University, Andong, Republic of Korea)
A growth inhibition assay using Chlorella sp. AG 10002 based on the OECD 201 standard test procedure was applied to the toxicity testing of endosulfan and its reported metabolites. Comparison of dry cell weight, optical density (OD) at 680 nm, and chlorophyll a concentration indicated that optical density at 680 nm of culture broth is convenient, rapid, and accurate method for cell growth. In this microalgae system, the IC∧50 values of endosulfan, endosulfan sulfate, endosulfan lactone, and endosulfan ether were determined as 9.45, 18.8, 18.2 and 37.5 mg/L, respectively.
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