Kinetic Properties of Extracted Lactate Dehydrogenase and Creatine Kinase from Mouse Embryonic Stem Cell- and Neonatal-derived Cardiomyocytes
2006
Zonouzi, Roseata (Islamic Azad University, Iran) | Ashtiani, Saeid Kazemi (Islamic Azad University, Iran) | Hosseinkhani, Saman (Tarbiat Modaress University, Tehran, Iran) | Baharvand, Hossein (Islamic Azad University, Iran), E-mail: [email protected]
Embryonic stem cells (ESCs), representing a population of undifferentiated pluripotent cells with both self-renewal and multilineage differentiation characteristics, are capable of spontaneous differentiation into cardiomyocytes. The present study sought to define the kinetic characterization of lactate dehydrogenase (LDH) and creatine kinase (CK) of ESC- and neonatal-derived cardiomyocytes. Spontaneously differentiated cardiomyocytes from embryoid bodies (EBs) derived from mouse ESC line (Royan B1) and neonatal cardiomyocytes were dispersed in a buffer solution.
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