Determination of Cytoplasmic Male Sterile Factors in Onion Plants (Allium cepa L.) Using PCR-RFLP and SNP Markers
2006
Cho, K.S. (National Institute of Highland Agriculture, RDA, Pyeongchang, Republic of Korea), E-mail: [email protected] | Yang, T.J. (National Institute of Agricultural Biotechnology, RDA, Suwon, Republic of Korea) | Hong, S.Y. (National Institute of Highland Agriculture, RDA, Pyeongchang, Republic of Korea) | Kwon, Y.S. (National Institute of Highland Agriculture, RDA, Pyeongchang, Republic of Korea) | Woo, J.G. (National Horticultural Research Institute, RDA, Suwon, Republic of Korea) | Park, H.G. (Seoul National University, Seoul, Republic of Korea)
We have developed a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) marker that can distinguish male-fertile (N) and male-sterile (S) cytoplasm in onions. The PCR-RFLP marker was located in a chloroplast psbA gene amplicon. Digesting the amplicons from different cytoplasm-containing varieties with the restriction enzyme MspI revealed that N-cytoplasm plants have a functional MspI site (CCGG), whereas the S-cytoplasm plants has a substitution in that site (CTGG), and thus no MspI target.
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