Isolation of a Novel Gellan-Depolymerizing Bacillus sp. Strain YJ-1
2006
Jung, Y.J. (Pusan National University, Busan, Republic of Korea) | Park, C.S. (Kyung Hee University, Yongin, Republic of Korea) | Lee, H.G. (Hanyang University, Seoul, Republic of Korea) | Cha, J.H. (Pusan National University, Busan, Republic of Korea), E-mail: [email protected]
A novel microorganism that could degrade high molecular weight gellan was screened and isolated from soil. On gellan plate, the microorganism grew well and completely liquefied the plate. The gellan-degrading microorganism was isolated by pure culture on glucose and nutrient agar medium afterwards. The 16S rDNA sequence analysis and biochemical tests using an API 50CHB/20E kit revealed that the strain belonged to Bacillus sp. The isolate, named as Bacillus sp. YJ-1, showed optimum gellan-degrading activity in 0.5% gellan medium at pH 7.5 and 37℃.
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