Construction of Conjugative Gene Transfer System Between E. coli and Moderately Thermophilic, Extremely Acidophilic Acidithiobacillus caldus MTH-04
2007
Liu, Xiangmei (Shangdong University, Jinan, P.R. China), E-mail: [email protected] | Lin, Jianqun (Shangdong University, Jinan, P.R. China) | Zhang, Zheng (Shangdong University, Jinan, P.R. China) | Bian, jiang (Shangdong University, Jinan, P.R. China) | Zhao, Qing (Shangdong University, Jinan, P.R. China) | Liu, Ying (Shangdong University, Jinan, P.R. China) | Lin, Jianqiang (Shangdong University, Jinan, P.R. China) | Yan, Wangming (Shangdong University, Jinan, P.R. China)
A genetic transfer system for introducing foreign genes to biomining microorganisms is urgently needed. Thus, a conjugative gene transfer system was investigated for a moderately thermophilic, extremely acidophilic biomining bacterium, Acidithiobacillus caldus MTH-04. The broad-host-range IncP plasmids RP4 and R68.45 were transferred directly into A. caldus MTH-04 from Escherichia coli by conjugation at relatively high frequencies. Additionally the broad-host-range IncQ plasmids pJRD215, pVLT33, and pVLT35 were also transferred into A. caldus MTH-04 with the help of plasmid RP4 or strains with plasmid RP4 integrated into their chromosome, such as E. coli SM10.
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