Molecular Cloning and Expression of Grass Carp MyoD in Yeast Pichia pastoris
2007
Wang, Lixin (Pearl River Fisheries Research Institute, CAFS. Key Lab. of Tropical and Subtropical Fish Breeding and Cultivation of CAFS, Guangzhou, P. R. China) | Bai, Junjie (Pearl River Fisheries Research Institute, CAFS. Key Lab. of Tropical and Subtropical Fish Breeding and Cultivation of CAFS, Guangzhou, P. R. China), E-mail: [email protected] | Luo, Jianren (Pearl River Fisheries Research Institute, CAFS. Key Lab. of Tropical and Subtropical Fish Breeding and Cultivation of CAFS, Guangzhou, P. R. China) | Chen, Hong (Northwest Science and Technology University of Agriculture and Forestry, Yangling, Shaanxi, P. R. China) | Ye, Xing (Pearl River Fisheries Research Institute, CAFS. Key Lab. of Tropical and Subtropical Fish Breeding and Cultivation of CAFS, Guangzhou, P. R. China) | Jian, Qing (Pearl River Fisheries Research Institute, CAFS. Key Lab. of Tropical and Subtropical Fish Breeding and Cultivation of CAFS, Guangzhou, P. R. China) | Lao, Haihua (Pearl River Fisheries Research Institute, CAFS. Key Lab. of Tropical and Subtropical Fish Breeding and Cultivation of CAFS, Guangzhou, P. R. China)
MyoD, expressed in skeletal muscle lineages of vertebrate embryo, is one of muscle-specific basic helix-loop-helix (bHLH) transcription factors, which plays a key role in the determination and differentiation of all skeletal muscle lineages. In this study, a cDNA of grass carp MyoD was cloned and characterized from total RNA of grass carp embryos by RT-PCR. The full-length cDNA of grass carp MyoD is 1597 bp. The cDNA sequence analysis reveals an open reading frame of 825 bp coding for a protein of 275 amino acids, which includes a bHLH domain composed of basic domain (1-84∨th amino acids) and HLH domain (98-142∨th amino acids), without signal peptide.
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