Enhancement of Erythropoietin Production in Recombinant Chinese Hamster Ovary Cells by Sodium Lactate Addition
2007
Choi, Y.S. (Korea University, Seoul, Republic of Korea) | Lee, D.Y. (Korea University, Seoul, Republic of Korea) | Kim, I.Y. (Korea University, Seoul, Republic of Korea) | Kim, H.J. (Chung-Ang University, Seoul, Republic of Korea) | Park, H.W. (Hanyang University, Seoul, Republic of Korea) | Choe, T.B. (Konkuk University, Seoul, Republic of Korea) | Kim, I.H. (Korea University, Seoul, Republic of Korea), E-mail: [email protected]
The stabilization of optimum pH for cells can cause a higher erythropoietin (EPO) production rate and a good growth rate with the prolonged culture span in recombinant Chinese hamster ovary (r-CHO) cells. Our strategy for stabilizing the optimum pH in this study is to reduce the lactate production by adding sodium lactate to a culture medium. When 40 mM sodium lactate was added, a specific growth rate was decreased by approximately 22% as compared with the control culture. However, the culture longevity was extended to 187 h, and more than a 2.7-fold increase in a final accumulated EPO concentration was obtained at 40 mM of sodium lactate.
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