Enhancing the bioremediation potential of chlorococcum humicola (Chlorophyta) Dil. 01 exposed to cadmium in altered NPS of BG-11 medium
2006
Cordero, C. | Perez, T. | Rivero, G., Pamantasan ng Lungsod ng Maynila, Intramuros, Manila (Philippines). Dept. of Biology
Growth of chlorococcum humicola Dil. 01, an isolate from the University of the Philippines, Diliman, Quezon City [Philippines], was enhanced through exposure to manipulation of the NPS content of the BG-11 medium. A total of 26 NPS combinations were achieved by either doubling or reducing the half the original concentrations of these three macronutrients. The cultures grown in the media with N 2P 2S and N 2P S supported the highest cell densities, while the cultures grown in the media with N 0.5 P 2 S and N 0.5 P S had the least densities. The bioremediation potential of C. humicola was also evaluated by exposure to 3 concentrations of CdCl2, i.e. 0.07, 0.7 and 7 ppm, for 72 hours. A significant amount of cadmium was found bund to the cell wall compared to that detected in the protoplast. The cultures in the medium 0.5 N 2P S exhibited the highest cell density values, while 2N 0.5 P 0.5S, the lowest during the toxicity testing period. The cultures in the medium with 0.5N P 0.5S accumulated the highest amount of cadmium, while the cultures in the medium with 2N 0.5P 0.5S, the least amount, which was detected in both the cell wall and the protoplast fractions. The data on cell density and on the amount of accumulated cadmium, in both the cell wall and protoplast, revealed that the medium with 0.5 N P 2S was the best formulation which enhanced the bioremediation potential of C. humicola, pertinent to the uptake of cadmium.
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