Phenotypic characterization and micropropagation of candidate PRSV resistant papaya lines from Agrobacterium-mediated transformation
2007
Magdalita, P.M. | Oropesa, A.M.R. | Valencia, L.D. | Tarun, J.A.M. | Hautea, V.M., Philippines Univ. Los Banos, College, Laguna (Philippines). Inst. of Plant Breeding, Crop Science Cluster
The phenotypic characteristics of three T2 (approximately F3 generation) candidate PRSV resistant lines and the 'Davao Solo' control grown in a contained BL2 facility were evaluated. Plant height, stem diameter, internode length, number of nodes and number of days to flower were the vegetative traits studied. Fruit weight, total soluble solids, percent edible portion, and average number of seeds were the quantitative traits evaluated. In addition, fruit shape, peel color and texture, flesh and seed color were the qualitative characters analyzed. The phenotypic traits of the transgenic candidate lines are within the range of the untransformed 'Davao Solo' control. Futhermore, the candidate lines and the control were micropropagated using the single nodal cutting technique for line maintenance and multiplication. A De Fossard medium supplemented with alpha-naphthalene acetic acid and 6-benzylaminopurine was used as multiplication medium while, a De Fossard supplemented with 3-indole-butyric acid was used as rooting medium. In vitro plantlets of these lines free of off-types were produced.
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