Genetic diversity assessment of finger millet, Eleusine coracana (Gaertn), germplasm through RAPD analysis
2004
Fakrudin, B. (University of Agricultural Sciences, Dharwad, (India). Department of Biotechnology) | Kulkarni, R.S. | Shashidhar, H.E. | Hittalmani, S.
Random amplified polymorphic DNA (RAPD) was used to evaluate genetic diversity among 12 selected finger millet accessions, representing different geographic origins and pedigree backgrounds. A genomic survey, employing 37 RAPD primers, detected 254 unambiguous, repeatable fragments with an average of 6.86 amplified fragments per primer. A large number of fragments (218), representing 85.82% of the total, were polymorphic; however, individual RAPD primers differed widely in their ability to detect polymorphism. As expected, the genetic diversity observed in African landraces was higher than that observed in Indian cultivated accessions, indicating that wild finger millet accessions contain a large proportion of genetic variation. Further, lack of polymorphism in the highly bred accessions strengthens the hypothesis of genetic erosion during breeding selection. Cluster analysis based on the unweighted pair-group method using arithmetic average clearly indicated apportionment of finger millet accessions in concordance with geographic origin and pedigree history
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