Bacteriological characteristics of Xanthomonas campestris pv. phaseoli bean pathogen in Serbia and possibilities of control: [Doctoral dissertation]
2006
Todorovic, B.
Xanthomonas campestris pv. phaseoli, causal agent of common blight is economically the most important bacterial disease of bean in all growing regions of this crop. After diseased plant samples collected and analysed, it was determined that these disease symptoms were caused by phytopathogenic bacteria. In order to identify isolated bacteria, their pathogenic, morphological, cultural, biochemical and physiological characteristics were studied. Based on the results of these investigations, it was determined that 30 investigated strains belonged to X.c.pv. phaseoli. Molecular identification of X.c.pv.phaseoli was done using polymerase chain reaction (PCR) based on amplification of 730 bp products in the genome of investigated strains. Serological studies, done by using PTA ELISA revealed that investigated strains react with antibodies specific for X.c.pv. phaseoli. Studies on resistance to X.c.pv. phaseoli in commercially grown bean genotypes in Serbia revealed that a high level of resistance to bacterial blight does not exist. In our study, bean cultivars: Dvadesetica, Prelom and Oplenac showed the highest sensitivity to this pathogen, while cultivar Oreol was resistant in both experiments. Combination of Dithane M-70 and Funguran OH exhibited the highest efficacy in the control of X.c.pv. phaseoli among the bactericide compounds applied in the study.
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