Secondary regeneration from somatic embryos of Aesculus carnea Hayne
2006
Zdravkovic-Korac, S.,Institute for Biological Research Sinisa Stankovic, Belgrade (Serbia) | Calic-Dragosavac, D.,Institute for Biological Research Sinisa Stankovic, Belgrade (Serbia) | Budimir, S.,Institute for Biological Research Sinisa Stankovic, Belgrade (Serbia) | Janosevic, D.,Faculty of Biology, Belgrade (Serbia). Institute of Botany and Botanical Garden Jevremovac | Vinterhalter, B.,Institute for Biological Research Sinisa Stankovic, Belgrade (Serbia) | Radojevic, Lj.,Institute for Biological Research Sinisa Stankovic, Belgrade (Serbia)
Somatic embryos of red horse chestnut were subjected to an induction of secondary regeneration. The embryos were divided in 4 classes on the basis of their size, and sub-cultivated on MS media containing 0, 1, 5 or 10 microM kinetin (Kin) or benzyladenine (BA). The pathway of secondary regeneration (somatic embryogenesis or caulogenesis) depended solely on the primary somatic embryo (PSE) stage of development. The PSE of the 1st and 2nd class produced solely secondary somatic embryos (SSE), the 3rd class PSE formed SSE on media containing Kin, and both SSE and adventitious buds on media containing BA, whereas the 4th class PSE developed almost solely adventitious buds. BA promoted bud induction at much higher rate than Kin and slighly higher enbryogenic response. The histological study confirmed these findings.
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