Effect of cold pretreatment on androgenous ability of wheat anthers
2008
Ljevnaic, B., Poljoprivredni fakultet, Novi Sad (Serbia) | Kondic-Spika, A., Naucni institut za ratarstvo i povrtarstvo, Novi Sad (Serbia)
In this paper the effect of cold pretreatment on androgenous ability of wheat anthers was investigated. Anthers were isolated from 12 different genotypes of wheat (Triticum aestivum L.). Before isolation, a half of anthers were exposed to a cold pretreatment on 4 +/- 1 deg C for 5-10 days, while the other half of anthers were inoculated onto the Potato-2 induction medium without the cold pretreatment. The results have shown that absence of cold pretreatment had significant effect on androgenous capacity in 5 cultivars, while on callus yield had the effect in 4 from the total of 12 examined wheat genotypes. It can be concluded that the effect of cold pretreatment on androgenous ability of wheat is genotype specific.
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