Effect of divalent ions on ruminal enzime activities in sheep
2006
Faixova, Z., Faculty of Veterinary Medicine, Kosice (Slovak Republic) | Faix, S., Academy of Sciences, Kosice (Slovak Republic) | Makova, Z., Faculty of Veterinary Medicine, Kosice (Slovak Republic) | Vaczi, P., Faculty of Veterinary Medicine, Kosice (Slovak Republic) | Prosbova, M., Faculty of Veterinary Medicine, Kosice (Slovak Republic)
The effect of different salts of divalent ions as potential inhibitors and stimulators of ruminal alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyltransferase (GGT), and glutamate dehydrogenase (GDH) activities in sheep was assessed using biochemical assays. Ruminal fluids was obtained from six cannulated ewes. Effects of ZnCl2, CuCl2, MgCl2, BaCl2, and CoCl2 on ruminal enzyme activites were tested after incubation of ruminal fluid in the presence of the salts at 37 deg C for 30 min. Two different concentrations of salts of divalent ions were tested. The results of the study indicate that the effect of divalent ions on ruminal enzyme activities depend on their relative concentration and which enzyme is assayed.
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