The determination of salinomycin residues in the tissues of broiler chickens by using microbiological diffusion methods
2002
Cabadaj, R., University of Veterinary Medicine (Slovakia) | Nagy, J. | Popelka, P. | Mate, D. | Bugarsky, A.
Two hundred three-day-old broiler chickens (ROSS hybrid) were used in the experiment. Salinomycin (Synvertas, Biotika Slovenska L’upca, The Slovak Republic) was orally administered to three groups of chickens in dosages of 600 mg/kg-1 of feed (60 mg salinomycin - group I); 900 mg/kg-1 of feed (90 mg salinomycin - group II); 1,800 mg/kg-1 of feed (180 mg salinomycin - group III). Sacox 120 (12 % salinomycin, Biotika Slovenska L’upca) was administered to the fourth group of chickens (500 mg/kg-1 feed; 60 mg of salinomycin). The birds were treated until they were 42 days old. Then 70 chickens were randomly selected and killed; 6 birds from each experimental group (I-IV) on the first day after the end of the treatment and two birds from each experimental group on the 2nd, 3rd, 4th, 5th, 6th and 7th days. Breast and thigh muscles, liver, gizzard and heart samples were analysed microbiologically, with Bacillus stearothermophillus var. calidolactis C 953 as a test micro-organism, and by the four-plate method. The four-plate method was more sensitive than the Bac. stearothermophillus microbiological method to the residues of salinomycin, which were detectable in the chicken tissues for between 4 and 6 days, depending on the dosage that they received.
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