Immunohistochemistry and canine skin and mammary gland tumours
2006
Sevcikova, Z. | Levkut, M. | Puzder, M. | Sevcik, A.
This study was focused on the use of selected antibodies in the differentiation of spontaneous canine skin and mammary gland tumours. The collective immunohistochemicall expression of 8.00 (low molecular weight -- LMW) and 8.60 (high molecular weight -- HMW) anticytokeratines peptide, desmin, vimentin, melanoma HMB 45 antibody, p53 tumours suppressor protein (DO-1), CD3 antiserum to human T-cells, S-100 protein, lysozyme, alpha-1 antitrypsinand proliferation cell nuclear antigen (PCNA) were analysed on formalin-fixed, paraffin-embedded and frozen tissue sections. The detection of vimentin, HMW cytokeratin,p53, CD3 cells, lysozyme and PCNA were the most useful.There was a less consistent and lighter positive response to desmin in all tumours. The reaction response to LMW cytokeratin was very low. The use of S-100 protein HMB 45, alpha-1 antitrypsin antibody as markers in canine skin and mammary gland tumours is not recommended. The findings in this study indicate that immunohistochemistry applied to tumours of unknown cellular origin is of a great potentional diagnostic benefit in veterinary pathology. But the usefulness of the immunohistochemical detection of tumours by different types of markers will be only shown when these stains have been standardised among many laboratories and a large body of information concerning these staining patterns has become available.
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