Lactate dehydrogenase isoenzyme pattern in serum of cattle at slaughter
2007
Heinová, D, Univerzita veterinárskeho lekárstva, Košice (Slovak Republic) | Vaško, L.
Lactate dehydrogenase (L-lactate: NAD oxidoreductase, EC 1.1.1.27) (LDH) isoenzymes pattern in serum of cattle at slaughter was investigated. An extraordinarily intensive diffuse zone was found at the cathodic site of LDH4 by gradient polyacrylamide gel electrophoresis.71% of total LDH activity was concentrated in this zone while the catalytic intensity of LDH1 to LDH3 decreased proportionaly (4.4-, 3.2- and 1. -times, respectively). This profile of serum lactate dehydrogenase isoenzymes was greatly different from that of the normal. Similar patterns were observed in some human serum/plasma where diffused LDH zones were identified as macroglobular complexes of lactate dehydrogenase isoenzymes with immunoglobulins. Using polyacrylamide gel isoelectric focusing technique in a pH range of 3.0 to 9.0 revealed that the diffused LDH zone had not been homogeneous. It consisted of two to four extra fractions which were localized in a relatively high and narrow pH area of 8.1 to 9.0. Their localization on the isoelectrophoretogram was different from that of the true LDH isoenzymes molecules with pI values of 4.2 to 6.7. As the extra LDH fractions occurred in sera of animals at slaughter we presume a relationship to the stress exercise.
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