Cytoskeleton, endoplasmic reticulum and nucleus alterations in CHO-K1 cell line after Crotalus durissus terrificus (South American rattlesnake) venom treatment
Tamieti, B. P.(Vale do Paraíba University Institute of Research and Development Laboratory of Cell and Tissue Biology) | Damatta, R. A.(State University of Norte Fluminense Center for Biosciences and Biotechnology Laboratory of Cell and Tissue Biology) | Cogo, J. C.(Vale do Paraíba University Institute of Research and Development Laboratory of Physiology and Pharmacology) | Da Silva, N. S.(Vale do Paraíba University Institute of Research and Development Laboratory of Cell and Tissue Biology) | Mittmann, J.(Vale do Paraíba University Institute of Research and Development Laboratory of Molecular Biology and Immunobiologics) | Pacheco-Soares, C.(Vale do Paraíba University Institute of Research and Development Laboratory of Cell and Tissue Biology)
Snake venoms are toxic to a variety of cell types. However, the intracellular damages and the cell death fate induced by venom are unclear. In the present work, the action of the South American rattlesnake Crotalus durissus terrificus venom on CHO-K1 cell line was analyzed. The cells CHO-K1 were incubated with C. d. terrificus venom (10, 50 and 100g/ml) for 1 and 24 hours, and structural alterations of actin filaments, endoplasmic reticulum and nucleus were assessed using specific fluorescent probes and agarose gel electrophoresis for DNA fragmentation. Significant structural changes were observed in all analyzed structures. DNA fragmentation was detected suggesting that, at the concentrations used, the venom induced apoptosis.
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