EFFECT OF THE MULTIENZYME PRODUCT HOSTAZYM C100 ON THE DIGESTION AND FEEDING BEHAVIOR OF BUFFALO CALVES FED ON DIETS WITH DIFFERENT PROPORTIONS OF CONCENTRATE AND ROUGHAGE. II. EFFECT OF THE MULTIENZYME PRODUCT HOSTAZYM C100 ON THE CELLULOLYTIC ACTIVITY, THE TOTAL NUMBER AND THE GENETIC STRUCTURE OF RUMEN INFUZORIA
2008
Slavov, T., Thracian University, Stara Zagora (Bulgaria) | Sivkova, K., Thracian University, Stara Zagora (Bulgaria) | Radev, V., Thracian University, Stara Zagora (Bulgaria) | Nedeva, N., Thracian University, Stara Zagora (Bulgaria)
The aim of this study was to determine the effect of the polyenzyme product Hostazym C100 on the total number and genetic structure of infuzoria, and celulolytic activity in the rumen fluid of buffalo calves fed with diets with different proportions of concentrate mixture and roughage. The experiment was carried out in four periods (two controls and two experimental ones), with five 8-month buffalo calves of the Bulgarian Murra breed with live weights of 165 kg at the beginning of the study. The animals were reared in individual boxes, in closed barns with permanent access to drinking water. The consumed feed during the first and the second period was composed of 2 kg concentrate mixture and 4 kg alfalfa hay. During the third and the fourth period the experimental animals were given 2.1 kg concentrate mixture and 7 kg alfalfa hay daily. In the experimental periods the enzyme product Hostazym C100 was added to the concentrate at a dose of 1g /kg mixture. Hostazym C100 has endo-1,4 beta glucanase basic activity and cellulase, alpha amylase, protease and hemicellulase secondary enzymatic activities. It was found that the inclusion of Hostazym C100 in the first diet (4 kg alfalfa hay and 2 kg concentrate) decreased the total number of infuzoria 2.5 hours after feeding (P0.05). In both diets the Hostazym C100 supplement reduced the percentage of Entodinium sp. infuzorians and increased those of Epidinium sp. and Isotricha sp. infuzorians. The enzymatic product reduced the cellulolytic activity in the first hours of the incubation (4, 6, 8 hours).
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