Influences of cefotaxime and carbenicillin on plant regeneration from wheat mature embryos
2008
Yu, Y.,Chinese Academy of Sciences, Shanghai (China). Inst. of Plant Physiology and Ecology | Wei, Z.M.,Chinese Academy of Sciences, Shanghai (China). Inst. of Plant Physiology and Ecology
Five wheat genotypes were used to study the influences of cefotaxime and carbenicillin on the regeneration potential of mature embryos. Filter-sterilized cefotaxime enhanced regeneration capacity although it did not affect the average number of shoots per explant. The highest regeneration capacity (55.4%) was obtained on regeneration medium supplemented with 100 mg/cubic dm cefotaxime. Filter-sterilized carbenicillin did not stimulate plant regeneration. However, higher concentration (100 mg/cubic dm) accelerated callus browning and inhibited the following regeneration. Autoclaved antibiotics at all tested concentrations showed detrimental effects on callus morphogenesis and plant regeneration.
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