Genetic diversity of five Chinese goat breeds assessed by microsatellite markers
Li, J.Y.,Northwest A and F Univ., Yangling (China). Shaanxi Key Lab. of Molecular Biology for Agriculture | Chen, H.,Northwest A and F Univ., Yangling (China). Shaanxi Key Lab. of Molecular Biology for Agriculture | Lan, X.Y.,Northwest A and F Univ., Yangling (China). Shaanxi Key Lab. of Molecular Biology for Agriculture | Kong, X.J.,Northwest A and F Univ., Yangling (China). Shaanxi Key Lab. of Molecular Biology for Agriculture | Min, L.J.,Qingdao Agricultural Univ. (China). Dept. of Animal Science
The objective of this research was to evaluate the genetic diversity in Laoshan dairy goat, Xinong Saanen dairy goat, Guanzhong dairy goat, Banjiao goat and Guizhou white goat using microsatellite DNA markers. Within the nine polymorphic loci, allele frequencies, number of effective alleles (Ne), heterozygosity (He), polymorphism information content (PIC), genetic identity (I) and Nei's standard genetic distance (D) were calculated. UPGMA phylogenetic tree was constructed based on allele frequencies. The average number of alleles was 9.4, ranging from four to eleven at the nine assessed loci. The average values of Ne, He, PIC of all loci were 4.716, 0.765, 0.732, respectively. The Guanzhong population showed the highest variability (PIC=0.78, He=0.80). There was a relatively high level of genetic diversity in the breeds studied. A UPGMA diagram, based on Nei's standard genetic distances, yielded relationships between populations that agreed with what is known about their origin, history and geographical distribution.
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