The differences in cell wall composition in leaves and regenerating protoplasts of Beta vulgaris and Nicotiana tabacum
Wisniewska, E.,Plant Breeding and Acclimatization Inst., Bydgoszcz (Poland). Lab. of Biotechnology | Majewska-Sawka, A.,Plant Breeding and Acclimatization Inst., Bydgoszcz (Poland). Lab. of Biotechnology
Cell wall composition in leaves and regenerating leaf-derived protoplasts was studied in Beta vulgaris and Nicotiana tabacum. Several oligosaccharides that characterize arabinogalactan proteins (AGPs) and pectins were visualized in situ by a series of immunocytochemical reactions. The most conspicuous difference between the two species involved the expression of AGP epitopes that bind LM2 and MAC207 in only sugar beet cells; both epitopes being characterized by the presence of glucuronic acid (GlcA). Moreover, B. vulgaris leaves and protoplast-derived cells showed lower content of pectin side-chains bearing (1-4)-beta-D-galactose residues as compared with N. tabacum.
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