Antioxidative response to cadmium in roots and leaves of tomato plants
Ammar, W.B.,Faculte des Sciences de Tunis, Tunis El Manar (Tunisia). Unite de Recherche Nutrition et Metabolisme Azotes et Proteines de Stress | Nouairi, I.,Centre de Biotechnologie de Borj-Cedria B, Hammam-Lif (Tunisia). Lab. Caracterisation et Qualite de l'Huile d'Olive | Zarrouk, M.,Centre de Biotechnologie de Borj-Cedria B, Hammam-Lif (Tunisia). Lab. Caracterisation et Qualite de l'Huile d'Olive | Ghorbel, M.H.,Faculte des Sciences de Tunis, Tunis El Manar (Tunisia). Unite de Recherche Nutrition et Metabolisme Azotes et Proteines de Stress | Jemal, F.,Faculte des Sciences de Tunis, Tunis El Manar (Tunisia). Unite de Recherche Nutrition et Metabolisme Azotes et Proteines de Stress
Treatment of tomato seedlings (cv. 63/5 F1) with increasing CdCl2 concentrations in the culture medium resulted in Cd accumulation which was more important in roots than in leaves. Biomass production was severely inhibited, even at low Cd concentration. Cd reduced chlorophyll content in leaves and enhanced lipid peroxidation. An increase in the activity of antioxidative enzymes was observed. The increase in the activity of superoxide dismutase, ascorbate peroxidase, guaiacol peroxidase, and glutathione reductase was more pronounced in leaves than in roots, while catalase activity increased only in roots. In addition, changes in isoenzyme composition were observed using the non-denaturing polyacrylamid gel electrophoresis.
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