Immunoglobulin producing cells in chickens immunized with Eimeria tenella gametocyte antigen vaccines
Ayaz, M.M.,University of Agriculture, Faisalabad (Pakistan). Dept. of Parasitology | Akhtar, M.,University of Agriculture, Faisalabad (Pakistan). Dept. of Parasitology | Hussain, I.,University of Agriculture, Faisalabad (Pakistan). Dept. of Parasitology | Muhammad, F.,University of Agriculture, Faisalabad (Pakistan). Dept. of Parasitology | Haq, A.U.,University of Agriculture, Faisalabad (Pakistan). Dept. of Parasitology
The present paper reports on the IgA, IgG and IgM antibodies secreting cells (ASC) in the spleen of chickens vaccinated with E. tenella (local isolates) gametocyte vaccine(s) by using Enzyme Linked Immunospot (ELISPOT) assay. Irrespective of the vaccine used, the number of IgG ASC in the spleen of orally vaccinated chickens was higher than the number of IgA and IgM ASC. Maximum numbers of IgG, IgA and IgM ASC were found in chickens vaccinated with sonicated gametocyte formalin inactivated vaccine (Group III) followed by formalin inactivated gametocytes (Group II) and gametocytes (Group I). The number of ASC (IgG, IgA and IgM) per 104 cells in spleen was significantly higher in Group III than in the two other groups. Results of the challenge experiments revealed maximum protection against mixed species of the genus Eimeria in Group III (77.8%), followed by Group II (55.6%) and Group I (52.0%). Lesion scoring was directly proportional to the mortality and oocysts per gram of droppings but inversely proportional to the protection. In conclusion, the spleen in chickens is one of the major sources of cells producing IgA, IgG and IgM antibodies.
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