Identification of heterosomes in spermatoza of rams with 54,XX/54,XY chimerism
2008
Kozubska-Sobocinska, A.,National Research Inst. of Animal Production, Balice/Krakow (Poland) | Rejduch, B.,University of Rzeszow, Kolbuszowa n. Rzeszow (Poland). Dept. of Biotechnology
The aim of the study was to identify heterosomes in the semen of three Romanov rams - carriers of leukocyte chimerism (FISH technique) and to determine the proportions between spermatozoa with X and Y chromosomes. The choice of bovine probes for hybridization with ram heterosomes was dictated by genetic conservatism of bovine and ovine heterosomes. The ratio between spermatozoa with a yellow fluorescent signal containing the X chromosome in the haploid set and spermatozoa with a red-purple signal indicating the presence of the Y chromosome, taking into account spermatozoa with no signal, was 52%:43%:5%, 47%:44%:9% and 48%:46%:6% in three rams studied. The results obtained lead us to conclude that the 54,XX/54,XY chimerism has no effect on sex ratio in offspring.
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