Chemical and microbiological characterization of Cambisols, Luvisols and Stagnosols
Ruzek, L.,Ceska Zemedelska Univ., Prague (Czech Republic). Fakulta Agrobiologie, Potravinovych a Prirodnich Zdroju | Ruzkova, M.,Ceska Zemedelska Univ., Prague (Czech Republic). Fakulta Agrobiologie, Potravinovych a Prirodnich Zdroju | Vorisek, K.,Ceska Zemedelska Univ., Prague (Czech Republic). Fakulta Agrobiologie, Potravinovych a Prirodnich Zdroju | Kubat, J.,Vyzkumny Ustav Rostlinne Vyroby, Prague (Czech Republic) | Friedlova, M.,Vyzkumny Ustav Rostlinne Vyroby, Prague (Czech Republic) | Mikanova, O.,Vyzkumny Ustav Rostlinne Vyroby, Prague (Czech Republic)
Cambisols, Luvisols and Stagnosols are the main soil taxonomical units covering 78% of the total area of the Czech Republic. In the period 2001-2008 soil samples from 13 arable and 2 grassed localities were collected and tested. Microbial biomass carbon (MBC) was determined using microwave irradiation method (MW), chloroform fumigation extraction method (FE) and re-hydration technique (RHD). Soil samples were moistened according to the content of organic carbon (Corg) by deionized water addition (%Corg x 0.19 mL/g DM - dry mass of soil) immediately before MBC determination. Microwave sterilization (800 J/g DM = 600 W, 2 x 67 s, 100 g DM (10 soil samples) and microwave soil extracts digestion (800 J/mL = 250 W, 77 s, 24 mL) give the lowest values of MBC (204+/-67 mg/kg DM; 100%) in comparison with FE (236+/-57 mg/kg DM; 116%) and RHD (478+/-138 mg/kg DM; 235%), respectively. High significant correlation (r = 0.9713) was found between total carbon (TC, 1.36+/-0.29%) and total nitrogen (TN, 0.15+/-0.03%) determined by CNS analyzer. Furthermore, high significant correlations were found between MBC-MW and MBC-RHD (r = 0.8965) as well as MBC-FE and dehydrogenase activity (DHA, r = 0.8094), respectively. DHA in studied soils reached 147+/-68 mg of triphenylformazan/kg DM/24 h. Corg formed 96% of TC and total Kjeldahl nitrogen 97% of TN, respectively. According to our results MW is fully acceptable for MBC determination.
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