The occurrence of enterotoxigenic isolates of B. cereus in foodstuffs
Brychta, J.,Statni Veterinarni Ustav, Jihlava (Czech Republic) | Smola, J.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Mikrobiologie a Imunologie | Pipek, P.,Vysoka Skola Chemicko-technologicka, Prague (Czech Republic). Ustav Konzervace Potravin a Technologie Masa | Ondracek, J.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Mikrobiologie a Imunologie | Bednar, V.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Mikrobiologie a Imunologie | Cizek, A.,Vysoka Skola Chemicko-technologicka, Prague (Czech Republic). Ustav Konzervace Potravin a Technologie Masa | Brychta, T.,Vysoka Skola Chemicko-technologicka, Prague (Czech Republic). Ustav Konzervace Potravin a Technologie Masa
Enterotoxigenic Bacillus cereus was detected in a variety of meat stuffs (36), ready-to-cook products (5), and swabs (7). The bacterial colonies isolated from PEMBA agar were identified as B. cereus. The 85 isolates were examined for the enterotoxin production using both TECRA-VIA and BCET-RPLA kits (ELISA - 47, RPLA - 38). Thirty two isolates (66%) were positive for enterotoxin using the ELISA test while only 15 isolates (39%) gave positive results in the RPLA test system. In total, 91.8% and 84% of the strains isolated in our laboratory were enterotoxigenic as determined using TECRA-VIA and BCET-RPLA, respectively. Parallel enterotoxin positive results obtained using both tests were demonstrated in only 9 isolates from 19 assessed. Coincidental negative results from both kits were established for 3 isolates only. The isolates of B.cereus from meat were resistant to cephalothin (57%), clindamycin (14%), oxytetracycline (14%), and erythromycin (7%). The isolates from swabs were resistant to cephalothin (83%), erythromycin (16%), clindamycin (16%) and enrofloxacin (16%).
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