Bioassy and studying stability of chitinase transgene in the different generations (T1 and T2) of transgenic cotton plants.
2008
Tuhid Far, Mas`ud
Study of the gene inheritance and expression is of great importance in gene transformation programs and, in fact, the outcome of research relies on it. In this study, transgenic cottons containing chitinase gene(antifungal) where the expression of gene had already been confirmed in them, stability of chitinase gene was evaluated using PCR, southern and western blot in the T1 and T2 transgenic plant and were bioassaed. Five seed clumps collected from the first generation (T0 ) of transgenic offsprings with one seed clump (as control) were used as the experimental treatments. PCR analysis revealed the presence of the chitinase gene in 77 out of 128 plants. Southern hybridization confirmed the integration of gene (chitinase) and western blot detected the experession of 32kd protein in transgenic plants. In study the effect of transgenic cotton plants (containing the bean chitinase gene) and untransgenic on the fungus V.dahalie it was revealed that transgenic and untransgenic cotton extracts had the most and least effects on fungus growth. From 8 transgenic lines under study at the T2 generation 3 lines were homozigous(AA) and 5 lines heterozigous(Aa). In order to investigation resistant to V. deahlia seedling infects with a spore suspension. Comparision of disease symbol showed that disease vertical cutting of stem showed that severity of vascular browing in non-transgenic more than transgenic plants. Also, growth average four week after inoculation showed that mean heights transgenic plants more than non-transformed plants.
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