Seminested PCR for Diagnosis of Neospora caninum Infection in Cattle
2005
Habibi, G.R., Razi Vaccine & Serum Research Institute, Tehran, iran | Hashemi-Fesharki, R., Razi Vaccine & Serum Research Institute, Tehran, iran | Sadrebazzaz A., Razi Vaccine & Serum Research Institute, Mashhad, iran | Bozorgi, S., Razi Vaccine & Serum Research Institute, Tehran, iran | Bordbar, N, Razi Vaccine & Serum Research Institute, Tehran, iran
Neospora caninum, an apicomplexan protozoan, is regarded as a major cause of abortion and stillbirth in cattle in countries world-wide. The ability to detection N.caninum in tissue samples can be a useful detection diagnostic tool for use in the study of the pathogenicity, immunoprophylaxis, and treatment of Neospora infection. However, molecular biology is one of the most sensitive tools for detecting protozoa in infected tissue samples. Specific semi-nested PCR was designed based on specific ITS1 and 5.8S rRNA genomic DNA for detection of parasite in infected tissues. The designed PCR detected four of six aborted fetal brain samples infected by N.caninum. Our results revealed that PCR with selected primers gave a 357bp product in examined samples and confirmed the presence of N.caninum DNA in infected fetal brains. This is the first report that demonstrated the reliability of PCRbased assay to identify N.caninum infection in Iran.
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