Identification of salt tolerance candidate genes in rice at repoductive stage
2007
Hoseyni Salekdeh, Qasem | Dadashi, A`li Asghar
Rice (Oryza sativa) is most sensitive to salinity during reproductive stage. We employed a proteomic approach to further understand the mechanism of plant responses to salinity at early reproductive stage. Plants were grown in culture solution and salt stress imposed at panicle initiation. After 12 days of stress, young panicles were collected from control and salt stressed plants. The Na+ and K+ content of panicle and several yield components changed significantly in response to short-term salt stress. The collected panicles were sorted in three different sizes (7±1, 11±1 and 15±1 mm) and their proteome patterns were analyzed using 2DE in triplicates. The expression pattern of 13 proteins significantly changed in all panicle sizes in response to stress. Mass spectrometry analysis of salt responsive proteins and 16 other highly abundant proteins of panicle led to identification of proteins involved in several salt responsive mechanisms which may increase plant adaptation to salt stress including higher constitutive expression level and up-regulation of antioxidants, up-regulation of proteins involved in translation, transcription, signal transduction, and ATP generation. To our knowledge, this is the first proteome analysis of plant young panicle which may enhance our understanding of plant molecular responses to salinity. Proteome reference map of rice young panicle is available at http://www.proteome.ir
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