Chromosome doubling of odd number polyploid strawberry [Fragaria ananassa] and characteristics of the resulting line
2008
Sasaki, M.(Shizuoka-ken. Research Inst. of Agriculture and Forestry, Iwata (Japan)) | Takeuchi, T.
This study investigated the various characteristics (growth, fertility, fragrant constituent, et cetera) of lines obtained by doubling the chromosomes of an odd number polyploid strawberry that was excellent in fragrance, but had low fertility. First, we obtained doubling individuals at a high rate by treating an odd number polyploid '97-20-1' (5x) with 200 ppm colchicine for 30 days. Second, in spite of an individual thinking as a complete doubling individual when we confirmed polyploidy after colchicine treatment, the chimera individual exited in individual multiplied by runner renewal. Thus, it is necessary to confirm polyploidy or growth again after runner renewal. Third, the fertility of pollen and fruit rose by doubling the odd number polyploid which originally had low fertility. But the fertility rate differed based on the colchicine treatment conditions. Thus, when we treat chromosomes with colchicine, it is necessary to think about the appropriate concentration or number of treatment days. We think that it is best to us a colchicine treatment of 100 ppm for 10 days based on the results of this study, considering doubling efficiency, growth and fruit fertility et cetera. Last, by doubling the fragrant hybrid which had low fertility, their fertility rose, furthermore, their fragrant constituents were inherited. Thus, it may be possible to breed a fragrant variety.
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