Generation of monoclonal antibody against canine neural-cell adhesion molecule
2008
Uematsu, Y.(Canine-Lab. Inc., Tokyo (Japan)) | Yamaguchi, T. | Koike, A. | Yagihara, H. | Hasegawa, D. | Matsuki, N. | Ono, K. | Washizu, T. | Arai, T. | Bonkobara, M.
A monoclonal antibody, K9BYU, was generated using Escherichia coli recombinant extracellular domain of canine neural-cell adhesion molecule (N-CAM) as an antigen. Immunoreactivity of K9BYU to insect cell recombinant canine N-CAM was demonstrated by Western blotting using Sf9 insect cells transfected with the canine N-CAM gene. In Western blotting against canine brain tissue, K9BYU detected three isoforms of N-CAM that correspond to three major isoforms of human and mouse N-CAM (N-CAM-120, -140, and -180). From these results, K9BYU was considered to be a useful tool for research of canine N-CAM.
Показать больше [+] Меньше [-]