A novel enzymatic method for measuring mizoribine 5'-monophosphate levels in serum
Ota, H.(Asahi Kasei Pharma Crop., Izunokuni, Shizuoka (Japan)) | Yasuda, Y. | Sakasegawa, S. | Imamura, S. | Tamura, T.
Inosine 5'-monophosphate dehydrogenases (IMPDH) from Oceanobacillus iheyensis and Bacillus subtilis were biochemically characterized with the aim of establishing a mizoribine 5'-monophosphate (MZR-P) assay. MZR-P is the active metabolite of mizoribine, which has been successfully used as an immunosuppressive agent. A sensitive method for measuring the MZR-P concentration in serum, based on its ability to inhibit IMPDH, was developed. The method was applied to an automatic clinical analyzer, enabling measurement of MZR-P levels in a set of serum samples. MZR-P at concentrations of 0.1-13.6 (microg/ml (0.3-40microM) in the samples could be measured with 0.7% within-run coefficients of variation.
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