Characterization of a flavin reductase from a thermophilic dibenzothiophene-desulfurizing bacterium, Bacillus subtilis WU-S2B
Takahashi, S.(Waseda Univ., Tokyo (Japan). Faculty of Science and Engineering) | Furuya, T. | Ishii, Y. | Kino, K. | Kirimura, K.
Bacillus subtilis WU-S2B is a thermophilic dibenzothiophene (DBT)-desulfurizing bacterium and produces a flavin reductase (Frb) that couples with DBT and DBT sulfone monooxygenases. The recombinant Frb was purified from Escherichia coli cells expressing the frb gene and was characterized. The purified Frb exhibited high stability over wide temperature and pH ranges of 20-55degC and 2-12, respectively. Frb contained FMN and exhibited both flavin reductase and nitroreductase activities.
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