Detection of bracken gene in gut content using a polymerase chain reaction
2009
Kamiyoshi, T.(Toyama-ken. Tobu Livestock Hygiene Service Center (Japan)) | Kozakura, R. | Okabe, T. | Tsubokawa, T.
It is essential for the diagnosis of bovine bracken poisoning to confirm that the cow has eaten bracken. Detection of the gene for the large subunit of the ribulose-bisphosphate carboxylase (rbcL) of pteridophytes in the gut contents from a Japanese black cow that died suddenly after depasturage was carried out using a polymerase chain reaction. PCR products were successfully amplified and one of them was analyzed phylogenetically. The sequence of PCR product from rumen content was identical to the Pteridium aquilinum gene in the same cluster. This PCR method will be helpful in diagnosing bovine bracken poisoning.
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