Effects of Growth Regulators and Sucrose Concentrations on Proliferation of In Vitro Shoot Using Bioreactor Culture in Zingiber officinale
2007
Ahn, J.H. (Buyeo Tomato Experiment Station, Chungnam ARES, Buyeo, Republic of Korea) | Lee, J.J. (Honam Agricultural Research Institute, NICS, RDA, Iksan, Republic of Korea) | Kim, T.S. (Honam Agricultural Research Institute, NICS, RDA, Iksan, Republic of Korea) | Kim, H.S. (Honam Agricultural Research Institute, NICS, RDA, Iksan, Republic of Korea) | Lee, S.Y. (Wonkwang University, Iksan, Republic of Korea), E-mail: [email protected]
In vitro shoot clusters of Zingiber officinale were initiated from the meristem culture on Murashinge and Skoog (MS) medium containing 0.1 mgㆍL-¹ NAA, 1 mgㆍL-¹ kinetin and 3% sucrose. After 4 weeks of bioreactor culture using adventitious shoots (10±3 mm size), the number of shoot per explant was the highest in MS medium containing 0.5 mgㆍL-¹ NAA, 2 mgㆍL-¹ BA and 3% sucrose (4.3 per explant), whereas the shoot height was significantly elongated in MS medium containing 0.1 mgㆍL-¹ NAA, 0.5 mgㆍL-¹ BA and 3% sucrose as compared to the others. When sucrose concentration was increased to 6% in MS media containing 0.1 mgㆍL-¹ NAA and 0.5 mgㆍL-¹ BA, both proliferation and growth of in vitro shoots were excellent without hyperhydricity. The result could provide the basic information for large-scale multiplication of in vitro shoot using the bioreactor culture.
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