Structural Analysis of Natural Indigo Colorants Extracted from Polygonum tinctorium
2007
Chung, I.M. (National Institute of Agricultural Science and Technology, RDA, Suwon, Republic of Korea), E-mail: [email protected] | Lee, K.G. (National Institute of Agricultural Science and Technology, RDA, Suwon, Republic of Korea) | Sung, G.B. (National Institute of Agricultural Science and Technology, RDA, Suwon, Republic of Korea) | Kim, H.B. (National Institute of Agricultural Science and Technology, RDA, Suwon, Republic of Korea) | Nam, S.H. (National Institute of Agricultural Science and Technology, RDA, Suwon, Republic of Korea) | Hong, I.P. (National Institute of Agricultural Science and Technology, RDA, Suwon, Republic of Korea)
Natural indigo colorants were prepared by extraction of Polygonum tinctorium which was harvested just in the blooming season (in the late of July). The components were analyzed by TLC and HPLC, and its structures were analyzed by FT-IR, EI-mass. The results obtained are summarized as follows; The natural indigo powder was dissolved in DMSO and developed in eluent, CHCI₃/CH₃CN (8.5 : 1.5 v/v) by means of TLC for its quality analysis. It was segregated into indirubin as a red colour and indigo as a blue colour. In case of HPLC analysis,. FT-IR spectrum of indirubin showed a peak for NH residue between 3200 and 3300 cm-¹. ¹H-NMR spectrum for indigo displayed AA'BB' spin system caused by indole structure between 6.5 and 7.7 ppm of H4, 5, 6 and 7, and -NH proton for indirubin showed an singlet between 10.88 and 11.0 ppm. EI-mass spectrum of indigo and indirubin both disclosed their molecular size as 262 and it implies that these two substances are isomer.
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