Production of carotenoids by β-ionone-resistant mutant of Xanthophyllomyces dendrorhous using various carbon sources
2008
Park, K.M. (Sungkyunkwan University, Suwon, Republic of Korea) | Song, M.W. (Sungkyunkwan University, Suwon, Republic of Korea) | Lee, J.H. (Korea University of Technology and Education, Cheonan, Republic of Korea), E-mail: [email protected]
Batch culture kinetics of the red yeast, Xanthophyllomyces dendrorhous SKKU 0107, revealed reduction in biomass with glucose and lower intracellular carotenoid content with fructose. Figures were different when compared to sucrose, which is a disaccharide of glucose and fructose. In contrast, specific growth rate constant stayed between 0.094~0.098 h-¹, irrespective of the carbon sources employed. Although the uptake rate of glucose was found to be 2.9-fold faster than that of fructose, sucrose was found to be a more suitable carbon source for the production of carotenoids by the studied strain. When sugar cane molasses was used, both the specific growth rate constant and the intracellular carotenoid content decreased by 27 and 17%, respectively. Compared with the batch culture using 28 g/L sugar cane molasses, fed-batch culture with the same strain resulted in a 1.45-fold higher cell yield together with a similar level of carotenoid content in X. dendrorhous SKKU 0107.
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