Detection of Microbial Growth in an Automated Culture System
2008
Sung, H.R. (Chungbuk National University, Cheongju, Republic of Korea) | Kim, I.H. (Chungbuk National University, Cheongju, Republic of Korea) | Kim, J.Y. (Chungbuk National University, Cheongju, Republic of Korea) | Lee, C.K. (Chungbuk National University, Cheongju, Republic of Korea) | Chung, Y.B. (Chungbuk National University, Cheongju, Republic of Korea) | Han, S.B. (Chungbuk National University, Cheongju, Republic of Korea) | Song, S.G. (Chungbuk National University, Cheongju, Republic of Korea), E-mail: [email protected]
Modern automated culture systems have increased the isolation rate of microorganisms and shortened the time to detection, reducing experimental errors in diagnosis of infecting agents. BacT/ALERT 3D system is based on the colorimetric detection of CO₂ produced by the growing microorganisms. In order to evaluate the efficiency of the detection system, sterility test were performed using 6 bacteria. With standard aerobic and anaerobic bottles containing the liquid media, both three aerobic bacteria (P. aeruginosa, M. luteus, B. subtilis) and a facultative bacterium S. aureus were detected up to 1 CFU in 31.44 hr. In addition, growth of anaerobic C. sporogenes was recognized up to 1 CFU in 15.96 hr. The slowly growing bacteria P. acnes was detected up to 10,000 CFU in 129.36 hr. In comparison with conventional culture method, BacT/ALERT 3D automated culture system was more sensitive and saved detection time up to 2~10 hr. Therefore, this automated culture system enables to efficiently detect bacteria in clinical samples and biological medicines.
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