Effect of non-enzymatic antioxidants in extender on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa
Andrabi, S.M.H. (National Agricultural Research Centre, Islamabad (Pakistan). Animal Sciences Inst.) | Ansari, M.S. (National Agricultural Research Centre, Islamabad (Pakistan). Animal Sciences Inst.) | Ullah, N. (National Agricultural Research Centre, Islamabad (Pakistan). Animal Sciences Inst.) | Afzal, M. (National Agricultural Research Centre, Islamabad (Pakistan). Animal Sciences Inst.)
The objective of the present study was to determine the effect of non-enzymatic antioxidants (vitamin C or E) in tris-citric acid buffer (TCA) on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa. Split pooled buffalo bull ejaculates were diluted in TCA egg yolk glycerol extender containing either vitamin C (TCAC), vitamin E (TCAE) or without antioxidant (TCAN) at 37 degree C. Extended semen was cooled to 4 degree C in 2 h and equilibrated for 4 h at 4 degree C. Cooled semen was then filled in 0.5 ml straws at 4 degree C and frozen in programmable cell freezer. Thawing of semen was performed at 37 degree C for 30 seconds. Sperm motility, plasma membrane integrity and sperm morphology (acrosome integrity, head, mid-piece and tail abnormalities) of each semen sample were evaluated. Percentage of post-thaw spermatozoal motility assessed visually at 0 and 6 h and the post-thaw percentage of spermatozoa with intact plasma membranes at 0 h were higher (P less than 0.05) with TCAC and TCAE compared to control. However, the differences in both these parameters between the former two groups were non-significant. The post-thaw percentage of spermatozoa with normal acrosomes was higher (P less than 0.05) in TCAE extender than control. Mean sperm abnormalities in samples cry preserved with extender having TCAC, TCAE or TCAN were similar (P greater than 0.05). In conclusion, non-enzymatic antioxidants, particularly vitamin E, in the tris-citric acid extender may improve the quality of frozen-thawed buffalo bull spermatozoa.
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