The influence of type and concentration of carbohydrates on growth and branching of Clematis integrifolia in vitro
Dabski, M. | Parzymies, M.,Akademia Rolnicza, Lublin (Poland). Inst. Roslin Ozdobnych i Architektury Krajobrazu
The influence of type and concentration of carbohydrates on in vitro shoot proliferation of Clematis integrifolia was studied. The explants used in the experiment were excised from aseptic cultures incubated on solidified MS medium. Shoot tips were placed on MS basal medium supplemented with 2 mg 2 iP/cubic dm and 0.5 mg IAA/cubic dm. The aim of the experiment was to estimate the influence of different carbohydrates: sucrose, galactose or fructose at 0, 10, 20, 30 or 40 g/cubic dm on the growth and proliferation of Clematis integrifolia explants. The results showed that fructose at 10 mg/cubic dm had the most positive effect on the growth of the main shoot. The highest proliferation rate was observed on the media supplemented with sucrose at 30 g/cubic dm and the obtained axillary shoots had the highest amount, length and weight. Galactose at all concentrations definitely inhibited growth and proliferation and rooting of shoots
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