The effect of chromium (III) on fatty acid metabolism and insulin path related gene expression in mouse myocytes cell line C2C12
2009
Lewicki, S. | Rattman, D. | Kuryl, T.,Szkola Glowna Gospodarstwa Wiejskiego, Warszawa (Poland). Katedra Nauk Fizjologicznych | Snochowski, M.,Polska Akademia Nauk, Jablonna (Poland). Inst. Fizjologii i Zywienia Zwierzat | Debski, B.,Szkola Glowna Gospodarstwa Wiejskiego, Warszawa (Poland). Katedra Nauk Fizjologicznych
The aim of the present study was to describe changes in beta-oxidation of fatty acids and gene expression following chromium suplementation. The experiments were performed in mouse myoblast C2C12 cell line over 4 days differentiation. Chromium was added to medium (DMEM), as chromium chloride or chromium picolinate, in 1 and 10 micro g Cr/L concentrations. Beta-oxidation of fatty acids activity was measured after 1, 3, 6 or 48 h incubations. The results in these studies showed positive effect of chromium suplementation on activity of beta-oxidation. Results from microarray analysis indicate chromium interaction on signaling insulin pathway, also on transcription level. Increased expression of genes engaged in lipid metabolism and genes activated by peroxisome proliferator-activated receptor PPAR suggest permanent effect caused by chromium ions
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