Detection of Colletotrichum acutatum latent infections in strawberry petioles and leaves | Utvrdjivanje prisustva Colletotrichum acutatum u latentno zarazenom liscu i lisnim drskama jagode
2008
Duduk, N., Univerzitet u Beogradu, Beograd - Zemun (Serbia). Poljoprivredni fakultet | Ivanovic, M., Univerzitet u Beogradu, Beograd - Zemun (Serbia). Poljoprivredni fakultet | Duduk, B., Institut za pesticide i zastitu zivotne sredine, Beograd (Serbia)
Investigation of the latent Colletotrichum acutatum infection was carried out on artificially inoculated strawberries. The initiation of fungi sporulation on symptomless petioles and leaves was carried out by exposing them to the herbicide paraquat (0.25%) and low temparatures, which caused plant tissue decay in different ways. Surface sterilization with 0.5% NaOCl precedes the exposure of plant material to paraquat. The freezing procedure was carried out by exposure of plant material to the temperature of -20 deg C for 2 h. After the freezing, one group was rinsed in Tween 20 (18 microl/l), and another group underwent surface steerilization in 0.0525% NaOCl with an addition of Tween 20 (18 microl/l). After 6 days of incubation, the appearance of acervuli and conidia was detected in 93.33 to 100% plant parts exposed to paraquat treatment and freezing procedure. In inoculated parts which were not exposed to herbicides or low temperatures, the presence of acervuli was detected in 3.33% tested petioles and 6.67% leaves.
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