Cloning and sequence analysis of gibberellin 20-oxidase gene in chestnut | 板栗GA20-氧化酶基因的克隆及序列分析
2009
Bao Xiaolan, Beijing University of Agriculture, Beijing(China), Department of Plant Science and Technology | Yang Kai, Beijing University of Agriculture, Beijing(China), New Technological Laboratory of Agricultural Application in Beijing | Feng Yongqing, Beijing University of Agriculture, Beijing(China), Department of Plant Science and Technology
Китайский. GA20-氧化酶（GA20-ox）是赤霉素生物合成中的限速酶，它能够催化从GA12到GA9及GA53到GA20过程中的3步氧化反应。笔者以板栗（Castanea mollissima Blume）为材料，应用基因组步移技术克隆GA20-ox基因，获得长度为2315bpDNA序列，其中含有一个GA20-ox基因的全长编码区序列，命名为CmGA200x1。CmGA200x1长度为1140bp，且编码区内存在2个内含子，其推定蛋白CmGA200x1序列长度为379个氨基酸，氨基酸序列比对分析显示CmGA200x1与山毛榉坚果的FsGA200x1（AJ420192）相似性为91.03％，系统发育树分析显示CmGA200x1与山毛榉坚果FsGA200x1（AJ420192）遗传距离最近。[著者文摘]
Показать больше [+] Меньше [-]Английский. Gibberellin 20-oxidase（GA20-ox）is an enzyme that can catalyses the three steps ,i. e. oxidization from GA12 to GA9 and GA53 to GA20 in the synthesis of active GAs and is a key point in the regulation of GA biosynthesis. Genomic walking was used to isolate GA20-ox gene from chestnut （Castanea mollissima Blume）and DNA sequence of 2315 bp was obtained, including entire coding region of one GA20-ox gene that was designated as CmGA20ox1. The entire coding region of CmGA20ox1 was 1 140 bp, including two introns or a deduced protein CmGA20ox1 of 379 amino acids,which had 91.03% similarity to FsGA20ox1 （AJ420192） from European beechnut （Fagus sylvatica L. ） analyzed by the amino acid sequence alignment, which showed the nearest genetic distance with FsGA20ox1（AJ420192）by the phylogenetic tree analysis.
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