PCR-RFLP and sequence analysis of Mx gene 3’ sequence in Langya chicken breeds | 琅琊鸡Mx基因3’端部分序列PCR-RFLP与序列分析
2009
Liu Zhenguo, Qingdao Agricultural University, Qingdao (China), College of Animal Science and Technology | Li Zhen, Qingdao Agricultural University, Qingdao (China), College of Animal Science and Technology | Wang Baowei, Qingdao Agricultural University, Qingdao (China), College of Animal Science and Technology
[Objective] The aim was to provide basis for molecular marker assisted selection and resistance breeding of Langya chicken. [Method] The genetic polymorphism of Hae Ⅲ site of Mx gene 3’ sequence in Langya chicken was analyzed by PCR-RFLP. [Result] The results showed that Hae Ⅲ site controlled by allele A and B were polymorphic in Langya chicken breeds and the allele frequencies of A and B were 0.562 and 0.438 respectively. The genotype distribution of Hae Ⅲ site was significantly not in Hardy-Weinberg equilibrium ( P0.01 )。 The polymorphic fragments were cloned and sequenced , and the results revealed that the fragment size was 357 bp and a deletion of 31 bp occurred in variation sequences. [Conclusion] It was found that Hae Ⅲ-RFLP exists in Mx gene 3’ sequence in Langya chicken breeds of Shandong Province.
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