The survival of Mycoplasma bovis at different temperatures
Vyletelova, M.,Agrovyzkum, Rapotin (Czech Republic)
The survival of Mycoplasma bovis in milk samples was investigated at three storage temperatures (5 deg C, -30 deg C and -80 deg C) during 5 weeks. For the storage temperatures -30 deg C and -80 deg C, the respective samples were prepared weekly and those for culture by repeated defrosting. At 5 deg C the total number of M. bovis CFU/mL decreased from the initial of 1.13 x 10*7 CFU/mL to the final of 3.92 x 10*6 CFU/mL. The development in the frozen samples prepared for each week was as follow: (1) at -30 deg C - from the initial of 1.13 x 10*7 CFU/mL to 5.69 x 10*6 CFU/mL; (2) at -80 deg C - from the initial of 1.13 x 10*7 CFU/mL to 9.75 x 10*6 CFU/mL. The decrease in M. bovis colony count was more evident in the samples that were repeatedly defrosted: (1) at -30 deg C - the initial of 1.13 x 10*7 CFU/mL to the final of 2.18 x 10*6 CFU/mL; (2) at -80 deg C - the initial and final values were 1.13 x 10*7 CFU/mL and 7.89 x 10*6 CFU/mL, respectively.
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