The contents of amino acids and sterols in maize plants growing under different nitrogen conditions
Pavlik, M.,Akademie Ved, Prague (Czech Republic). Ustav Experimentalni Botaniky | Pavlikova, D.,Ceska Zemedelska Univ., Prague (Czech Republic). Fakulta Agrobiologie, Potravinovych a Prirodnich Zdroju | Balik, J.,Ceska Zemedelska Univ., Prague (Czech Republic). Fakulta Agrobiologie, Potravinovych a Prirodnich Zdroju | Neuberg, M.,Ceska Zemedelska Univ., Prague (Czech Republic). Fakulta Agrobiologie, Potravinovych a Prirodnich Zdroju
The effect of nitrogen nutrition on phytosterol and amino acid content in aboveground biomass of maize was investigated in a pot experiment. N doses (2 or 4 g N/pot) were applied in the form of ammonium nitrate (AN) for control treatments or urea ammonium nitrate solution (UAN). UAN solution was applied according to Controlled Uptake Long Term Ammonium Nutrition method. The content of amino acids in plant biomass was affected by N nutrition. An increase of glutamine and asparagine levels in maize aboveground biomass was observed after UAN solution application. The results of free beta-sitosterol analyses by HPLC showed its 94% increasing concentration after UAN application in contrast to AN treatments. Our results confirmed that sterol interconversions are controlled by environmental conditions. They are involved in the regulation of membrane properties in response to changing growth conditions.
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